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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-BLAST is a tool that uses Primer3 and BLAST to design primers for PCR. You can enter a sequence, a range, or a refseq record as PCR template, and adjust various parameters to optimize primer specificity and performance.

   • Primer-BLAST

     Maximum number of database sequences (with unique sequence...

   • Tips for Finding Specific Primers

     Try "re-search for specific primers" feature on Primer-BLAST...

   • Retrieve Recent Jobs

     Retrieve recent primer-blast jobs. Enter a job id: Last...

   • Primer-BLAST Help

     Primer-BLAST: A tool to design target-specific primers for...

   • Publication

     BLAST and global alignment algorithm to screen primers...

   • PMC Free Article

     We present a new software tool called Primer-BLAST to...

   • PubMed

     Primer-BLAST allows users to design new target-specific...

2. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity

   www.ncbi.nlm.nih.gov › guide › howto

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   Primer BLAST is a tool that helps you design and evaluate PCR primers based on a target sequence or a template sequence. You can enter one or both primer sequences, select the organism and database, and get primer pairs and specificity information.

3. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 13:134. Use of Primer3 itself is subject to the terms and conditions stated on Primer3 web site .

4. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   BLAST and global alignment algorithm to screen primers against user-selected database. in order to avoid primer pairs (all combinations including forward-reverse primer pair, forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.

5. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

   www.ncbi.nlm.nih.gov › pmc › articles

   18 iun. 2012 · We present a new software tool called Primer-BLAST to alleviate the difficulty in designing target-specific primers. This tool combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.

6. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Try "re-search for specific primers" feature on Primer-BLAST result page. When only non-specific primers are found, the result page offers the option to re-search for specific primers.

7. pubmed.ncbi.nlm.nih.gov › 22708584Primer-BLAST: a tool to design target-specific primers for ... -...

   pubmed.ncbi.nlm.nih.gov › 22708584

   18 iun. 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.