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Primer3 was a complete re-implementation of an earlier program: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information.
Primer3 is an open source tool that helps you create primers for PCR, hybridization probes and sequencing. You can customize the input parameters to suit your goals and preferences.
Primer3Plus is a web tool that uses Primer3 algorithm to design primers and probes for PCR and sequencing. You can paste or upload a template sequence, set various parameters and constraints, and get a list of optimal primers and their properties.
Primer3Plus is a web-based application that allows you to design and optimize primers and probes for PCR, sequencing, and hybridization. You can paste or upload your sequence, mark the region of interest, and choose the parameters for primer selection and evaluation.
Primer3 was a complete re-implementation of an earlier program: Primer 0.5 ( Steve Lincoln, Mark Daly, and Eric S. Lander ). Lincoln Stein championed the idea of making Primer3 a software component suitable for high-throughput primer design. Web interface by Steve Rozen.
Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.
Primer3 is a widely used tool to select primers for polymerase chain reaction (PCR) from DNA sequence. It is written in C and C++ and can be installed from GitHub with instructions and manual.
