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  1. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Blast

      Blast - Basic Local Alignment Search Tool - BLAST

    • Protein Blast

      Protein Blast - Basic Local Alignment Search Tool - BLAST

    • Blastx

      Blastx - Basic Local Alignment Search Tool - BLAST

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

    • Blast FAQ

      To search only sequences for an organism or taxonomic group,...

    • Recent Results

      Enter a BLAST RID in the RID box below and click Go to find...

    • Blast Download Page

      The NCBI provides a suite of command-line tools to run BLAST...

  2. QuickBLASTP is an accelerated version of BLASTP that is very fast and works best if the target percent identity is 50% or more. BlastP simply compares a protein query to a protein database. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run.

  3. In order to run BLAST locally on your own computer, you will need to download the BLAST+ software and databases. To see the instructions and get the latest version of BLAST+, go to the BLAST download page.

  4. The Protein database is a collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB. Protein sequences are the fundamental determinants of biological structure and function.

  5. blast.ncbi.nlm.nih.gov › smartblastSmart BLAST

    Smart Blast searches a protein query against the landmark database. Enter Protein Query Sequence. Enter one protein accession, gi, or FASTA sequence [?] Clear. Show results in a new window.

  6. 6 aug. 2024 · BLASTx (translated nucleotide sequence searched against protein sequences): compares a nucleotide query sequence that is translated in six reading frames (resulting in six protein sequences) against a database of protein sequences.

  7. 17 mai 2017 · QuickBLASTP, an accelerated version of BLASTP, adds a new pre-processing step to the non-redundant (nr) protein database. In a matter of seconds, QuickBLASTP will find approximately 97% of the database sequences with 70% or more identity to your query and around 98% of the database sequence with 80% or more identity to your query.

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