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  1. Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information. For other services provided by ELIXIR's Estonian Node visit https://elixir.ut.ee/services .

    • Primer3 Output

      Primer3 Input on veebipõhine tööriist, mis võimaldab valida...

    • Version 0.4.0

      There is a newer version of Primer3 available at...

    • Primer3 Input

      A string to identify your output. Targets: E.g. 50,2...

    • Primer Task

      These output tags are intended to provide information on the...

  2. There is a newer version of Primer3 available at http://primer3.ut.ee. Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored). FASTA format ok.

  3. primer3.orgPrimer3

    Primer3 is a tool for designing PCR primers, hybridization probes and sequencing primers. It has many input parameters that you can control to optimize your primer design for your goals.

  4. INPUT AND OUTPUT CONVENTIONS. By default, Primer3 accepts input in Boulder-IO format, a pre-XML, pre-RDF, text-based input/output format for program-to-program data interchange. By default, Primer3 also produces output in the same format. When run with the --format_output command-line flag, Primer3 prints a more user-oriented report for each ...

  5. Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.

  6. These output tags are intended to provide information on the number of oligos and primer pairs that Primer3 examined and counts of the number discarded for various reasons. If -format_output is set similar information is produced in the user-oriented output. PRIMER_LIBERAL_BASE (boolean; default 0)

  7. 21 iun. 2012 · Primer3_core also generates its output in boulder IO format (Figure 4B). This format is designed for integration with other software, including scripts and web services, on the assumption that end users will interact with the web interfaces.

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